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With both bacterial and eukaryotic cells constantly dying and breaking down around us, and with a tremendous load of fragmentary DNA washing around in the fluid world, why aren't genes being transferred among various living organisms at a massive scale daily?


A) DNA that is not protected inside a protein coat or membrane is very vulnerable.
B) Living cells maintain a very effective barrier to the introduction of large molecules.
C) Since a vector appears necessary to snatch up DNA from one protected source and inject it into a cell, "naked" DNA is apparently vulnerable and unable to cross membranes easily.
D) All of the choices are correct.
E) None of the choices are correct, since DNA is readily exchanged among all organisms in nature.

F) B) and E)
G) A) and E)

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Gene therapy in humans has been used to treat


A) cystic fibrosis.
B) cancer.
C) inborn errors of metabolism.
D) All of the answer choices.
E) Only two of the answer choices.

F) A) and B)
G) A) and C)

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Gene pharming is the use of


A) transgenic plants to produce larger vegetables and sweeter fruits.
B) bacteria to produce organic compounds like aspartame and phenylalanine.
C) STR profiling to determine evolutionary relationships among organisms.
D) transgenic farm animals to produce pharmaceuticals.

E) B) and C)
F) C) and D)

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What is the first step in producing a transgenic animal?


A) implantation of a donor egg into the host mother
B) inject the desired gene(s) into a donor egg
C) transgenic organism produces offspring that have the desired gene which can then produce more transgenic offspring
D) transgenic eggs are harvested from the surrogate mother

E) A) and C)
F) C) and D)

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A transgenic organism is


A) one that contains a gene from another organism of the same or different species.
B) one produced by cloning a mutant cell.
C) one that acts as the donor for DNA to be moved into another organism.
D) one produced by the polymerase chain reaction.
E) any genetically modified organism resulting from laboratory research.

F) A) and E)
G) All of the above

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DNA fingerprinting may be used to establish paternity. Analyze the banding pattern to determine the father of the child. DNA fingerprinting may be used to establish paternity. Analyze the banding pattern to determine the father of the child.   A)  Male 1 B)  Male 2 The father of the child is Male 1, not Male 2.


A) Male 1
B) Male 2 The father of the child is Male 1, not Male 2.

C) A) and B)
D) undefined

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What consequences could arise if the field of bioinformatics ceased to exist as a field of study?


A) Progress in determining the function of DNA sequences, making comparisons between our genome and other model organisms, and understanding how genes and proteins interact with cells would stop.
B) Many cures for genetic disorders would not be discovered.
C) The creation of new transgenic plants, animals and bacteria that benefit society would not occur.
D) All of these are consequences if the field of bioinformatics ceased to exist as a field of study.

E) A) and C)
F) B) and D)

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When bone marrow stem cells are removed from the patient, infected with a virus that carries a normal gene, and returned to the patient, this is an example of


A) PCR.
B) chemotherapy.
C) viral disinfection.
D) in vivo gene therapy.
E) ex vivo gene therapy.

F) B) and E)
G) A) and D)

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Which of the following health disorders can be treated with ex vivo gene therapy?


A) Liver cells lack a protein that enables it to remove cholesterol from the blood which leads to high levels of cholesterol in the blood. This can increase the patients susceptibility to heart attacks.
B) The liver cells lack a protein that causes it to produce excessive cholesterol which leads to high levels of cholesterol in the blood. This can increase the patients susceptibility to heart attacks.
C) A gene is implanted that will code for the correct transmembrane carrier of the chloride ion.
D) Genes are implanted that make healthy cells more tolerant to chemotherapy while making the tumor cells more susceptible to chemotherapy.

E) All of the above
F) A) and B)

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Transgenic plant products include all of the following EXCEPT


A) soybeans that are resistant to a common herbicide.
B) an antibody produced by soybeans to treat genital herpes.
C) suicide genes that cause the plants to self destruct after their product has been produced.
D) antibodies produced by corn plants that deliver radioisotopes to tumor cells.

E) A) and D)
F) B) and C)

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To carry out a polymerase chain reaction (PCR) , you must have DNA polymerase and


A) a blueprint or gene map of the sequence you wish to copy.
B) the nucleotides to synthesize new DNA strands.
C) a DNA synthesizer machine.
D) a DNA probe.

E) A) and B)
F) A) and C)

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